Laboratory Methods In Microbiology And Molecular Biology

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Advanced Methods in Molecular Biology and Biotechnology

Advanced Methods in Molecular Biology and Biotechnology
  • Author : Khalid Z. Masoodi,Sameena Maqbool Lone,Rovidha Saba Rasool
  • Publisher : Academic Press
  • Release Date : 2020-11-10
  • Total pages : 218
  • ISBN : 9780128244500
  • File Size : 25,7 Mb
  • Total Download : 189
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Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work. This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level. Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology Features clear, step-by-step instruction for applying the techniques covered Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

Molecular Biology Techniques

Molecular Biology Techniques
  • Author : Heather Miller,D. Scott Witherow,Sue Carson
  • Publisher : Academic Press
  • Release Date : 2011-10-18
  • Total pages : 232
  • ISBN : 9780123855459
  • File Size : 39,8 Mb
  • Total Download : 349
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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions

Laboratory Methods in Microbiology and Molecular Biology

Laboratory Methods in Microbiology and Molecular Biology
  • Author : Richa Salwan,Vivek Sharma
  • Publisher : Elsevier
  • Release Date : 2023-03-01
  • Total pages : 0
  • ISBN : 9780323950794
  • File Size : 29,7 Mb
  • Total Download : 955
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Laboratory Methods in Microbiology and Molecular Biology: Methods in Molecular Microbiology is a unique, informative and multidisciplinary work that is not only confined to one discipline. It provides various procedures in diverse disciplines from microbiology to genetics, molecular biology and biochemistry. Here, the principles and facts underlying practical applications of bacteria and fungi which have prospects in various technologies are listed. It is composed of 15 chapters broadly covering basic concepts in microbiology, including soil microbiology, food microbiology, microbial genetics, serology and the use of basic bioinformatics tools. Each chapter contains a set of experiments with an introduction, principle, reagents and buffers used, brief methodology, conclusions and inferences, and a list of 20-40 references. A brief summary/abstract is also included. The book will facilitate students, teachers and researchers to monitor the precision and accuracy of their qualitative and quantitative methods practically. Involves various procedures in diverse disciplines, from microbiology to genetics, molecular biology and biochemistry Lists the principles and facts underlying practical applications of bacteria and fungi which have prospects in various technologies Includes the questions ‘how’ and ‘why’ as an explanation for novice students and researchers to modify protocols Facilitates students, teachers and researchers to monitor the precision and accuracy of their qualitative and quantitative methods practically

Molecular Microbiology Laboratory

Molecular Microbiology Laboratory
  • Author : Walt Ream,Bruce Geller,Katherine Field
  • Publisher : Academic Press
  • Release Date : 2012-08-31
  • Total pages : 322
  • ISBN : 9780123970442
  • File Size : 26,8 Mb
  • Total Download : 110
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"Intends to teach principles and techniques of molecular biology and microbial ecology to upper-level undergraduates majoring in the life sciences and to develop students' scientific writing skills. This title exposes students to the molecular-based techniques. It provides faculty with an accessible resource for teaching protocols."--WorldCat.

Laboratory Methods in Microbiology

Laboratory Methods in Microbiology
  • Author : W. F. Harrigan,Margaret E. McCance
  • Publisher : Academic Press
  • Release Date : 2014-06-28
  • Total pages : 374
  • ISBN : 9781483274348
  • File Size : 55,5 Mb
  • Total Download : 559
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Laboratory Methods in Microbiology is a laboratory manual based on the experience of the authors over several years in devising and organizing practical classes in microbiology to meet the requirements of students following courses in microbiology at the West of Scotland Agricultural College. The primary object of the manual is to provide a laboratory handbook for use by students following food science, dairying, agriculture and allied courses to degree and diploma level, in addition to being of value to students reading microbiology or general bacteriology. It is hoped that laboratory workers in the food manufacturing and dairying industries will find the book useful in the microbiological aspects of quality control and production development. The book is organized into two parts. Part I is concerned with basic methods in microbiology and would normally form the basis of a first year course. Abbreviated recipes and formulations for a number of typical media and reagents are included where appropriate, so that the principles involved are more readily apparent. Part II consists of an extension of these basic methods into microbiology as applied in the food manufacturing, dairying and allied industries. In this part, the methods in current use are given in addition to, or in place of, the ""classical"" or conventional techniques.

Current Protocols Essential Laboratory Techniques

Current Protocols Essential Laboratory Techniques
  • Author : Sean R. Gallagher,Emily A. Wiley
  • Publisher : Current Protocols
  • Release Date : 2012-03-19
  • Total pages : 664
  • ISBN : 047094241X
  • File Size : 15,9 Mb
  • Total Download : 527
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The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.

Molecular Biology Techniques

Molecular Biology Techniques
  • Author : Walt Ream,Katharine G. Field
  • Publisher : Academic Press
  • Release Date : 1998-11-17
  • Total pages : 234
  • ISBN : 9780080536828
  • File Size : 54,7 Mb
  • Total Download : 562
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This manual is designed as an intensive introduction to the various tools of molecular biology. It introduces all the basic methods of molecular biology including cloning, PCR, Southern (DNA) blotting, Northern (RNA) blotting, Western blotting, DNA sequencing, oligo-directed mutagenesis, and protein expression. Key Features * Provides well-tested experimental protocols for each technique * Lists the reagents and preparation of each experiment separately * Contains a complete schedule of experiments and the preparation required * Includes study questions at the end of each chapter

A laboratory Text book of Biochemistry, Molecular Biology and Microbiology

A laboratory Text book of Biochemistry, Molecular Biology and Microbiology
  • Author : Sharad Vats
  • Publisher : GRIN Verlag
  • Release Date : 2015-01-08
  • Total pages : 215
  • ISBN : 9783656871507
  • File Size : 44,8 Mb
  • Total Download : 136
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Document from the year 2014 in the subject Biology - Micro- and Molecular Biology, , language: English, abstract: A laboratory Text book of Biochemistry, Molecular Biology and Microbiology is intended to prepare the undergraduate, postgraduate and research students to perform basic experiments on various aspects of bioscience and biotechnology. Moreover, in the Semester system of teaching it is necessary to explore experiments which are not lengthy and easily completed within contact hours. Initially the book deals with dilutions, pH, buffers, units of measurements and calculations. This is followed by lab safety rules which is very important for any student working with chemicals for their and safety of others. This book emphasizes on principles, reagent preparations and procedures related to experiments, which will be handy for students from different scientific backgrounds. A number of methods are available in the literature for quantification of various molecules. This book does not present all the available methods but based on experience it contains commonly used methods, which students should know. The methods have been written in a manner for direct practical use in the laboratory. This work has originated as a result of numerous requests from my students for eased out and explanatory methods pertaining to biochemistry, biotechnology, microbiology and others. The section on testing of adulterants is of much use for common mass because most of the food products we eat are adulterated. The approach is rather simple with the use of very easily available chemicals and the tests can be performed even in house. It is hoped that the reliable assays presented in this manual will help the students and research scholars to get to basics of experiments and various aspects associated with it.

Principles and Techniques of Biochemistry and Molecular Biology

Principles and Techniques of Biochemistry and Molecular Biology
  • Author : Keith Wilson,John Walker
  • Publisher : Cambridge University Press
  • Release Date : 2010-03-04
  • Total pages : 769
  • ISBN : 9781107393493
  • File Size : 42,8 Mb
  • Total Download : 275
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This best-selling undergraduate textbook provides an introduction to key experimental techniques from across the biosciences. It uniquely integrates the theories and practices that drive the fields of biology and medicine, comprehensively covering both the methods students will encounter in lab classes and those that underpin recent advances and discoveries. Its problem-solving approach continues with worked examples that set a challenge and then show students how the challenge is met. New to this edition are case studies, for example, that illustrate the relevance of the principles and techniques to the diagnosis and treatment of individual patients. Coverage is expanded to include a section on stem cells, chapters on immunochemical techniques and spectroscopy techniques, and additional chapters on drug discovery and development, and clinical biochemistry. Experimental design and the statistical analysis of data are emphasised throughout to ensure students are equipped to successfully plan their own experiments and examine the results obtained.

Laboratory Methods in Enzymology: Protein

Laboratory Methods in Enzymology: Protein
  • Author : Anonim
  • Publisher : Academic Press
  • Release Date : 2014-02-24
  • Total pages : 240
  • ISBN : 9780124201798
  • File Size : 26,8 Mb
  • Total Download : 823
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Laboratory Methods in Enzymology: Protein Part B brings together a number of core protocols concentrating on protein, carefully written and edited by experts. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocols In this volume we have brought together a number of core protocols concentrating on protein

Laboratory Methods in Enzymology: RNA

Laboratory Methods in Enzymology: RNA
  • Author : Jon Lorsch
  • Publisher : Academic Press
  • Release Date : 2013-09-11
  • Total pages : 472
  • ISBN : 9780124200517
  • File Size : 55,5 Mb
  • Total Download : 501
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Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on RNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocols In this volume we have brought together a number of core protocols concentrating on RNA

Laboratory Manual of Microbiology, Biochemistry and Molecular Biology

Laboratory Manual of Microbiology, Biochemistry and Molecular Biology
  • Author : J. Saxena,M. Baunthiyal,I. Ravi
  • Publisher : Scientific Publishers
  • Release Date : 2015-05-01
  • Total pages : 380
  • ISBN : 9789386237231
  • File Size : 52,8 Mb
  • Total Download : 502
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Though many practical books are available in the market but this Laboratory Manual of Microbiology, Biochemistry and Molecular Biology is an unique combination of protocols that covers maximum (about 80%) of the practicals of various Indian universities for UG and PG courses in Bioscience, Biotechnology, Microbiology, Biochemistry and Biochemical Engineering.

Medical Mycology

Medical Mycology
  • Author : Kevin Kavanagh
  • Publisher : John Wiley & Sons
  • Release Date : 2006-08-14
  • Total pages : 348
  • ISBN : 9780470057407
  • File Size : 53,6 Mb
  • Total Download : 761
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Medical Mycology: Cellular and Molecular techniques is a clear and concise overview of the subject that details the techniques essential for ongoing research in the area. Drawing together contributions from both scientists and clinicians working in the field, the text will provide a valuable perspective on the applicability of specific techniques to patient care. A wide range of molecular, immunological and cytological techniques are discussed throughout, with the inclusion of protocol section in each chapter designed to provide both a background a up-to-date account of the applications of each procedure. Every technique is fully referenced and illustrations are provided where required to enhance student understanding. comprehensive introduction to the key techniques critical to the study of medical mycology clear explanation of how each technique is applied in the lab contributions from internationally recognised experts in the field outlines the background to many techniques required for the successful completion of a research project An invaluable reference for students of microbiology, biochemistry and molecular biology as well as postgraduates and researchers in the field of medical mycology looking for an up-to-date overview of the latest laboratory techniques.

Laboratory Practices in Microbiology

Laboratory Practices in Microbiology
  • Author : Osman Erkmen
  • Publisher : Academic Press
  • Release Date : 2021-02-06
  • Total pages : 292
  • ISBN : 9780323900157
  • File Size : 35,6 Mb
  • Total Download : 438
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Laboratory Practices in Microbiology provides updated insights on methods of isolation and cultivation, morphology of microorganisms, the determination of biochemical activities of microorganisms, and physical and chemical effects on microorganisms. Sections cover methods of preparation of media and their sterilization, microorganisms in environment, aseptic techniques, pure culture techniques, preservation of cultures, morphological characteristics of microorganisms, wet-mount and hanging-drop techniques, different staining techniques, cultural and biochemical characteristics of bacteria, antimicrobial effects of agents on microorganisms, hand scrubbing in the removal of microorganisms, characteristics of fungi, uses of bacteriophages in different applications, and more. Applications are designed to be common, complete with equipment, minimal expense and quick to the markets. Images are added to applications, helping readers better follow the expressions and make them more understandable. This is an essential book for students and researchers in microbiology, the health sciences, food engineering and technology, and medicine, as well as anyone working in a laboratory setting with microorganisms. Gives complete explanations for all steps in experiments, thus helping readers easily understand experimental procedures Includes certain subjects that tend to be disregarded in other microbiology laboratory books, including microorganisms in the environment, pure culture methods, wet-mount and hanging drop methods, biochemical characteristics of microorganisms, osmotic pressure effects on microorganisms, antiseptic and disinfectants effects on microorganisms, and more Provides groupings and characterizations of microorganisms Functions as a representative reference book for the field of microbiology in the laboratory

Analyzing Microbes

Analyzing Microbes
  • Author : Dilip Kumar Arora,Surajit Das,Mesapogu Sukumar
  • Publisher : Springer
  • Release Date : 2016-09-24
  • Total pages : 352
  • ISBN : 3662509679
  • File Size : 16,8 Mb
  • Total Download : 109
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This Springer Protocols manual is a practical guide to the application of key molecular biology techniques in microbiological research. The focus is on experimental protocols, which are presented in an easy-to-follow way, as step-by-step procedures for direct use in the laboratory. Notes on how to successfully apply the procedures are included, as well as recommendations regarding materials and suppliers. In addition to the practical protocols, important background information and representative results of experiments using the described methods are presented. Researchers in all areas applying microbial systems, such as in molecular biology, genetics, pathology, and agricultural research will find this work of great value.

Laboratory Methods in Cell Biology

Laboratory Methods in Cell Biology
  • Author : Anonim
  • Publisher : Academic Press
  • Release Date : 2012-12-31
  • Total pages : 544
  • ISBN : 9780124055490
  • File Size : 40,8 Mb
  • Total Download : 798
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Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as transfection, cell enrichment and magnetic batch separation. Covers the most important laboratory methods in cell biology Chapters written by experts in their fields

Methods for General and Molecular Microbiology

Methods for General and Molecular Microbiology
  • Author : C. A. Reddy,Terry J. Beveridge,John A. Breznak,George Marzluf
  • Publisher : American Society for Microbiology Press
  • Release Date : 2007-08-17
  • Total pages : 2737
  • ISBN : 9781555812232
  • File Size : 52,9 Mb
  • Total Download : 623
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A first source for traditional methods of microbiology as well as commonly used modern molecular microbiological methods. • Provides a comprehensive compendium of methods used in general and molecular microbiology. • Contains many new and expanded chapters, including a section on the newly important field of community and genomic analysis. • Provides step-by-step coverage of procedures, with an extensive list of references to guide the user to the original literature for more complete descriptions. • Presents methods for bacteria, archaea, and for the first time a section on mycology. • Numerous schematics and illustrations (both color and black and white) help the reader to easily understand the topics presented.

Basic Techniques in Biochemistry, Microbiology and Molecular Biology

Basic Techniques in Biochemistry, Microbiology and Molecular Biology
  • Author : Aakanchha Jain,Richa Jain,Sourabh Jain
  • Publisher : Humana
  • Release Date : 2021-03-14
  • Total pages : 282
  • ISBN : 1493998633
  • File Size : 52,7 Mb
  • Total Download : 310
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This book presents key methodologies, tools and databases for biochemistry, microbiology and molecular biology in simple and straightforward language. Covering all aspects related to experimental principles and procedures, the protocols included here are brief and clearly defined, and include essential precautions to be taken while conducting experiments. The book is divided into two major sections: one on constructing, working with, and standard operating procedures for laboratory instruments; and one on practical procedures used in molecular biology, microbiology and biochemical analysis experiments, which are described in full. Each chapter describes both the basic theory and relevant practical details for a given experiment, and helps readers recognize both the experiment’s potential and limitations. Intended as an intensive introduction to the various tools used in molecular biology, the book covers all basic methods and equipment, including cloning, PCR, spectrophotometers, ELISA readers, sonicators, etc. As such, it offers a valuable asset for final year undergraduate (especially project) students, graduate research students, research scientists and technicians who wish to understand and employ new techniques in the field of biotechnology.

Basic Techniques in Molecular Biology

Basic Techniques in Molecular Biology
  • Author : Stefan Surzycki
  • Publisher : Springer Science & Business Media
  • Release Date : 2012-12-06
  • Total pages : 434
  • ISBN : 9783642569685
  • File Size : 13,5 Mb
  • Total Download : 632
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This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.

Laboratory Methods in Enzymology: DNA

Laboratory Methods in Enzymology: DNA
  • Author : Anonim
  • Publisher : Elsevier
  • Release Date : 2013-09-02
  • Total pages : 408
  • ISBN : 9780124199545
  • File Size : 30,6 Mb
  • Total Download : 126
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Methods in Enzymology volumes provide an indispensable tool for the researcher. Each volume is carefully written and edited by experts to contain state-of-the-art reviews and step-by-step protocols. In this volume, we have brought together a number of core protocols concentrating on DNA, complementing the traditional content that is found in past, present and future Methods in Enzymology volumes. Indispensable tool for the researcher Carefully written and edited by experts to contain step-by-step protocols In this volume we have brought together a number of core protocols concentrating on DNA

Laboratory Techniques in Biochemistry and Molecular Biology

Laboratory Techniques in Biochemistry and Molecular Biology
  • Author : Thomas Spence Work,Elizabeth Work
  • Publisher : Unknown
  • Release Date : 1993
  • Total pages : 332
  • ISBN : UOM:39015026055247
  • File Size : 35,5 Mb
  • Total Download : 239
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PDF book entitled Laboratory Techniques in Biochemistry and Molecular Biology written by Thomas Spence Work,Elizabeth Work and published by Unknown which was released on 1993 with total hardcover pages 332, the book become popular and critical acclaim.