Introductory Experiments On Biomolecules And Their Interactions

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Introductory Experiments on Biomolecules and their Interactions

Introductory Experiments on Biomolecules and their Interactions
  • Author : Robert K. Delong,Qiongqiong Zhou
  • Publisher : Academic Press
  • Release Date : 2015-03-06
  • Total pages : 126
  • ISBN : 9780128010129
  • File Size : 32,9 Mb
  • Total Download : 966
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Introductory Experiments on Biomolecules and their Interactions provides a novel approach to teaching biomolecules in the lab. While featuring the requisite fundamentals, it also captures the author’s experience in industry, thus providing unique, up-to-date experiments which take the learning experience one-step further. The text parallels lectures using a standard biochemistry undergraduate text. Unlike most current lab manuals available in the market which simply emphasize an introduction of techniques, this lab manual provides students with opportunities to demonstrate and prove the knowledge and theories they learn from class. Features quantitative analysis of RNA degradation by RNase Contains problem sets, calculations, and references for each lab fully immersing students in the learning process Includes instruction on how to maintain a lab notebook and write a formal lab report Provides hands-on engagement with the four major types of biomolecules and “real-life and better applied examples of molecular interactions

An Introduction to Single Molecule Biophysics

An Introduction to Single Molecule Biophysics
  • Author : Yuri L. Lyubchenko
  • Publisher : CRC Press
  • Release Date : 2017-11-22
  • Total pages : 334
  • ISBN : 9781351333092
  • File Size : 22,8 Mb
  • Total Download : 937
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This book gives an accessible, detailed overview on techniques of single molecule biophysics (SMB), showing how they are applied to numerous biological problems associated with understanding the molecular mechanisms of DNA replication, transcription, and translation, as well as functioning of molecular machines. It covers major single molecule imaging and probing techniques, highlighting key strengths and limitations of each method using recent examples. The chapters begin with a discussion of single molecule fluorescence techniques followed by an overview of the atomic force microscope and its use for direct time-lapse visualization of dynamics of molecular complexes at the nanoscale, as well as applications in measurements of interactions between molecules and mechanical properties of isolated molecules and their complexes. The next chapters address magnetic tweezers and optical tweezers, including instrumentation, fundamentals of operation, and applications. A final chapter turns to nanopore transport and nanopore-based DNA sequencing technology that will play a major role in next-generation genomics and healthcare applications.

Real-Time Analysis of Biomolecular Interactions

Real-Time Analysis of Biomolecular Interactions
  • Author : K. Nagata,H. Handa
  • Publisher : Springer Science & Business Media
  • Release Date : 2013-12-11
  • Total pages : 256
  • ISBN : 9784431669708
  • File Size : 52,6 Mb
  • Total Download : 148
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The metabolism of all living organisms consists of elaborate and complex interactions among numbers of biomolecules, including protein-protein interactions. BIACORE is a new device that uses surface plasmon resonance for kinetic analysis of molecular interactions and automates whole analytical processes. The number of users of BIACORE is increasing worldwide, but until now nothing had been published in English to explain the principles of this new methodology and the kinds of applications it makes available. This updated and revised version of the Japanese original explains the underlying principles of BIACORE, providing concrete examples that utilize the methodology for analysis in areas ranging from basic to applied sciences. With its generous use of illustrations, this book is a valuable source of information for all users of BIACORE.

The Smallest Biomolecules: Diatomics and their Interactions with Heme Proteins

The Smallest Biomolecules: Diatomics and their Interactions with Heme Proteins
  • Author : Abhik Ghosh
  • Publisher : Elsevier
  • Release Date : 2011-10-13
  • Total pages : 614
  • ISBN : 0080556329
  • File Size : 38,8 Mb
  • Total Download : 873
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This is not a book on NO biology, nor about hemoglobin, nor about heme-based sensors per se. Of course, it covers all these topics and more, but above all, it aims at providing a truly multidisciplinary perspective of heme-diatomic interactions. The overarching goal is to build bridges among disciplines, to bring about a meeting of minds. The contributors to this book hail from diverse university departments and disciplines – chemistry, biochemistry, molecular biology, microbiology, zoology, physics, medicine and surgery, bringing with them very different views of heme-diatomic interactions. The hope is that the juxtaposition of this diversity will lead to increased exchanges of ideas, approaches, and techniques across traditional disciplinary boundaries. The authors represent a veritable Who’s Who of heme protein research and include John Olson, Tom Spiro, Walter Zumft, F. Ann Walker, Teizo Kitagawa, W. Robert Scheidt, Pat Farmer, Marie-Alda Gilles-Gonzalez, and many other equally distinguished scientists. Extremely distinguished list of authors Multidisciplinary character – equally suitable for chemists and biochemists Covers the hottest topics in heme protein research: sensors, NO biology, new roles of hemoglobin, etc.

Handbook of Biophotonics

Handbook of Biophotonics
  • Author : Jürgen Popp,Valery V. Tuchin,Arthur Chiou,Stefan H. Heinemann
  • Publisher : John Wiley & Sons
  • Release Date : 2011-11-30
  • Total pages : 1187
  • ISBN : 9783527410484
  • File Size : 40,9 Mb
  • Total Download : 772
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This new handbook covers the world of biophotonics not only geographically -- with the editors coming from different continents -- but also in terms of content, since the authors come from the whole spectrum of biophotonic basic and applied research. Designed to set the standard for the scientific community, these three volumes break new ground by providing readers with the physics basics as well as the biological and medical background, together with detailed reports on recent technical advances. The Handbook also adopts an application-related approach, starting with the application and then citing the various tools to solve the scientific task, making it of particular value to medical doctors. Divided into several sections, the first part offers introductory chapters on the different fields of research, with subsequent parts focusing on the applications and techniques in various fields of industry and research. The result is a handy source for scientists seeking the basics in a condensed form, and equally a reference for quickly gathering the knowledge from neighboring disciplines. Absolutely invaluable for biophotonic scientists in their daily work.

Introduction to Computational Health Informatics

Introduction to Computational Health Informatics
  • Author : Arvind Kumar Bansal,Javed Iqbal Khan,S. Kaisar Alam
  • Publisher : CRC Press
  • Release Date : 2020-01-08
  • Total pages : 664
  • ISBN : 9781000761597
  • File Size : 39,8 Mb
  • Total Download : 907
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This class-tested textbook is designed for a semester-long graduate or senior undergraduate course on Computational Health Informatics. The focus of the book is on computational techniques that are widely used in health data analysis and health informatics and it integrates computer science and clinical perspectives. This book prepares computer science students for careers in computational health informatics and medical data analysis. Features Integrates computer science and clinical perspectives Describes various statistical and artificial intelligence techniques, including machine learning techniques such as clustering of temporal data, regression analysis, neural networks, HMM, decision trees, SVM, and data mining, all of which are techniques used widely used in health-data analysis Describes computational techniques such as multidimensional and multimedia data representation and retrieval, ontology, patient-data deidentification, temporal data analysis, heterogeneous databases, medical image analysis and transmission, biosignal analysis, pervasive healthcare, automated text-analysis, health-vocabulary knowledgebases and medical information-exchange Includes bioinformatics and pharmacokinetics techniques and their applications to vaccine and drug development

Circular Dichroism and Linear Dichroism

Circular Dichroism and Linear Dichroism
  • Author : Alison Rodger,Bengt Nordén,University Lecturer Department of Chemistry Alison Rodger,Professor of Physical Chemistry Department of Physical Chemistry Bengt Nordén
  • Publisher : Oxford University Press, USA
  • Release Date : 1997
  • Total pages : 170
  • ISBN : 019855897X
  • File Size : 17,6 Mb
  • Total Download : 423
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This book provides an introduction to all those who wish to use the complementary spectroscopic techniques of optical activity (circular dichroism, CD) and optical anisotropy (linear dichroism, LD) for the study of the structure of molecules and interactions between molecules in solution. It emphasizes these techniques and how to use them for both low and high molecular weight molecules. The book begins by describing the principles behind CD and LD and how these techniques can be used in the laboratory without using advanced maths or quantum mechanics. The next chapters describe how both techniques may be applied to the study of biological macromolecules and give a detailed description of how they may be used on small molecules to investigate molecular and electronic structure. The final part contains theoretical derivations of all the equations required for the applications described previously. Specific molecular examples are used to illustrate concepts and to show the reader how to use the techniques in chemical and biological systems. Circular Dichroism and Linear Dichroism is an easy guide to what a prospective user of CD needs to know and explains how LD is not merely an exotic technique only to be practiced by experienced spectroscopists, but may be routinely and usefully employed as an aid to molecular structure determination.

Metallic Nanocrystallites and their Interaction with Microbial Systems

Metallic Nanocrystallites and their Interaction with Microbial Systems
  • Author : Anil K. Suresh
  • Publisher : Springer Science & Business Media
  • Release Date : 2012-03-02
  • Total pages : 67
  • ISBN : 9789400742314
  • File Size : 50,7 Mb
  • Total Download : 508
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Although interactions between nanoparticles and microorganisms in the environment are unavoidable and commonplace, it is still not clear what potential effects they may have. Metallic Nanocrystallites and their Interface with Microbial Systems not only illustrates how microbes and these particular nanoparticles interact but also it describes the consequences of these interactions. This brief discusses the impact of gold, silver, zinc oxide, and cerium oxide nanoparticles on the growth and viability of both Gram-negative and Gram-positive bacterium. Moreover, it analyses the relationship between bacterial growth inhibition, reactive oxygen species generation, the regulation of transcriptional stress genomes, and the toxicity of these materials. Finally, it reviews the specific metallic nanomaterials and highlights their modes of synthesis, reactivity at surfaces, and the importance of assay procedures in determining their toxicity levels. Various microscopy techniques used to determine their mechanisms of action are also presented. Metallic Nanocrystallites and their Interface with Microbial Systems will be a valuable source to the scientific and industrial community as well as to students and researchers in microbiology, biotechnology, nanotechnology, toxicology, materials science, biomedical engineering, cell and molecular biology.

Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods

Single-Molecule Enzymology: Fluorescence-Based and High-Throughput Methods
  • Author : Anonim
  • Publisher : Academic Press
  • Release Date : 2016-10-28
  • Total pages : 616
  • ISBN : 9780128095478
  • File Size : 23,7 Mb
  • Total Download : 654
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Single-Molecule Enzymology, Part A, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in single-molecule enzymology Contains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, nanopores, and tethered particle motion

NMR of Biological Macromolecules

NMR of Biological Macromolecules
  • Author : Chariklia I. Stassinopoulou
  • Publisher : Springer Science & Business Media
  • Release Date : 2013-06-29
  • Total pages : 357
  • ISBN : 9783642791581
  • File Size : 24,8 Mb
  • Total Download : 202
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Provided here are the latest techniques of NMR as applied to the study of proteins, carbohydrates and nucleic acids. The first chapters are devoted to an introduction to NMR and parameters related to molecular structure and molecular interactions. NMR experiments from basic 1D to 2D, 3D and 4D, used in combination with isotopically labelled molecules, are described and a general strategy is presented for biomacromolecular structure determination. Subsequent chapters deal with more advanced principles and techniques and their applications to structural and dynamic processes involving biomacromolecules in solution. Advanced results on peptide, protein, oligosaccharide and nucleic acid structure and recognition are presented.

Photophysics of Ionic Biochromophores

Photophysics of Ionic Biochromophores
  • Author : Steen Brøndsted Nielsen,Jean Ann Wyer
  • Publisher : Springer Science & Business Media
  • Release Date : 2013-10-16
  • Total pages : 230
  • ISBN : 9783642401909
  • File Size : 42,6 Mb
  • Total Download : 369
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This book provides a concise overview of the photophysics and spectroscopy of bio chromophore ions. The book "Photophysics of Ionic Biochromophores" summarizes important recent advances in the spectroscopy of isolated biomolecular ions in vacuo, which has within the last decade become a highly active research field. Advanced instrumental apparatus and the steady increase in more and more powerful computers have made this development possible, both for experimentalists and theoreticians. Applied techniques described here include absorption and fluorescence spectroscopy, which are excellent indicators of environmental effects and can thus shed light on the intrinsic electronic structures of ions without perturbations from e.g. water molecules, counter ions, nearby charges, and polar amino acid residues. When compared with spectra of the chromophores in their natural environment, such spectra allow to identify possible perturbations. At the same time gas-phase spectra provide important benchmarks for quantum chemistry calculations of electronically excited states. This volume focuses on biological systems from protein biochromophores, e.g. the protonated Schiff-base retinal responsible for vision, and individual aromatic amino acids to peptides and whole proteins, studied using visible, ultraviolet and vacuum ultraviolet light. Work on DNA nucleotides and strands that are amenable to mass spectrometric studies because of the negatively charged sugarphosphate backbone are also presented. DNA strands represent an example of the interplay between multiple chromophores, which is even harder to model correctly than just single chromophores due to spatially extended excited states and weak coupling terms. The experimental techniques used to measure spectra and commonly used theoretical methods are described with a discussion on limitations and advantages. The volume includes an updated status of the field and interesting future directions such as cold ion spectroscopy.

Biomolecules in Organic Solvents

Biomolecules in Organic Solvents
  • Author : Armando Gomez-Puyou
  • Publisher : CRC Press
  • Release Date : 1992-01-27
  • Total pages : 304
  • ISBN : 0849348234
  • File Size : 26,5 Mb
  • Total Download : 332
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Biomolecules and Organic Solvents discusses the behavior of enzymes and multi-enzyme complexes in organic solvents, in addition to the problem of water-protein interactions and the characteristics of water at interphases. It attempts to bridge the gap between the all water and the organic solvents systems from the point of view of enzyme structure and function. The mechanism of action of enzymes dispersed in anhydrous organic solvents and the biotechnological perspectives of the field are evaluated, and new information regarding the function and characteristics of complex multi-enzymatic systems and whole cells in organic solvents are examined. New developments regarding the nature of the water pool and interphases of reverse micelles and their enzyme kinetics are also explored. The characteristics and properties of enzymes trapped in reverse micelles are discussed throughout the book. Biomolecules in Organic Solvents is essential reading for biochemists, molecular biologists, and others working in related fields.

Quick Guideline for Computational Drug Design (Revised Edition)

Quick Guideline for Computational Drug Design (Revised Edition)
  • Author : Sheikh Arslan Sehgal,Rana Adnan Tahir,Muhammad Waqas
  • Publisher : Bentham Science Publishers
  • Release Date : 2021-09-16
  • Total pages : 126
  • ISBN : 9789814998703
  • File Size : 39,7 Mb
  • Total Download : 854
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Bioinformatics allows researchers to answer biological questions with advanced computational methods which involves the application of statistics and mathematical modeling. Structural bioinformatics enables the prediction and analysis of 3D structures of macromolecules while Computer Aided Drug Designing (CADD) assists scientists to design effective active molecules against diseases. However, the concepts in structural bioinformatics and CADD can be complex to understand for students and educated laymen. This quick guideline is intended as a basic manual for beginner students and instructors involved in bioinformatics and computational chemistry courses. Readers will learn the basics of structural bioinformatics, primary and secondary analysis and prediction, structural visualization, structural analysis and molecular docking. The book provides the reader an easy to read summary of the tools and techniques in structural bioinformatics as well as their limitations. In this revised edition, the authors have updated information in a number of chapters with a specific focus on the section on protein structure visualization and evaluation. Additional information on protein-ligand interaction studies has also been provided in this new edition. Therefore, the book is a useful handbook for aspiring scholars who wish to learn the basic concepts in computational analysis of biomolecules.

Physical Methods for Inorganic Biochemistry

Physical Methods for Inorganic Biochemistry
  • Author : John R. Wright,Wayne A. Hendrickson,Shigemasa Osaki,Gordon T. James
  • Publisher : Springer Science & Business Media
  • Release Date : 2012-12-06
  • Total pages : 400
  • ISBN : 9781468449976
  • File Size : 30,8 Mb
  • Total Download : 930
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This volume is intended for students and professionals in diverse areas of the biological and biochemical sciences. It is oriented to those who are unfamiliar with the use of physical methods in studies of the biological elements. We hope the reader will find the material a helpful reference for other volumes of this series as well as the general literature, and some may see ways to adopt these techniques in their own pursuits. Every effort has been made to avoid an abstruse presentation. It should be clear that one individual cannot be expert in all the disciplines considered here (and the authors recognize that fact with sin cere humility). As may be expected of an introductory reference, most of our attention was focused on the commonly used methods. To balance this, we have included a few examples of approaches which are promising but relatively undeveloped at this time. Also, an emphasis has been placed on element selectivity. It is impossible to envision the course of future events, and a volume which deals with instrumentation is especially prone to become outdated. Nevertheless, any valid approach to a scientific question should be applicable indefinitely.

Molecular Electronics: An Introduction To Theory And Experiment (2nd Edition)

Molecular Electronics: An Introduction To Theory And Experiment (2nd Edition)
  • Author : Elke Scheer,Juan Carlos Cuevas
  • Publisher : World Scientific
  • Release Date : 2017-05-19
  • Total pages : 848
  • ISBN : 9789813226043
  • File Size : 25,9 Mb
  • Total Download : 791
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Molecular Electronics is self-contained and unified in its presentation. It can be used as a textbook on nanoelectronics by graduate students and advanced undergraduates studying physics and chemistry. In addition, included in this new edition are previously unpublished material that will help researchers gain a deeper understanding into the basic concepts involved in the field of molecular electronics.

Book of abstracts PHOTONICA2017 The Sixth International School and Conference on Photonics & COST actions: MP1406 and MP1402 &H2020-MSCA-RISE-2015 CARDIALLY workshop

Book of abstracts PHOTONICA2017 The Sixth International School and Conference on Photonics & COST actions: MP1406 and MP1402 &H2020-MSCA-RISE-2015 CARDIALLY workshop
  • Author : Marina Lekić,Aleksandar Krmpot
  • Publisher : Institut za nuklearne nauke VINČA
  • Release Date : 2017-08-28
  • Total pages : 248
  • ISBN : 9788682441465
  • File Size : 14,7 Mb
  • Total Download : 354
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PDF book entitled Book of abstracts PHOTONICA2017 The Sixth International School and Conference on Photonics & COST actions: MP1406 and MP1402 &H2020-MSCA-RISE-2015 CARDIALLY workshop written by Marina Lekić,Aleksandar Krmpot and published by Institut za nuklearne nauke VINČA which was released on 2017-08-28 with total hardcover pages 248, the book become popular and critical acclaim.

Introduction to Biophysical Methods for Protein and Nucleic Acid Research

Introduction to Biophysical Methods for Protein and Nucleic Acid Research
  • Author : Jay A. Glasel,Murray P. Deutscher
  • Publisher : Academic Press
  • Release Date : 1995-11-20
  • Total pages : 510
  • ISBN : 0080534988
  • File Size : 53,6 Mb
  • Total Download : 465
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The first of its kind, Introduction to Biophysical Methods for Protein and Nucleic Acid Research serves as a text for the experienced researcher and student requiring an introduction to the field. Each chapter presents a description of the physical basis of the method, the type of information that may be obtained with the method, how data should be analyzed and interpreted and, where appropriate, practical tips about procedures and equipment. Key Features * Modern Use of Mass Spectroscopy * NMR Spectroscopy * Molecular Modeling and Graphics * Macintosh and DOS/Windows 3.x disks

Concepts of Biology

Concepts of Biology
  • Author : Samantha Fowler,Rebecca Roush,James Wise
  • Publisher : Unknown
  • Release Date : 2018-01-07
  • Total pages : 618
  • ISBN : 9888407457
  • File Size : 55,7 Mb
  • Total Download : 829
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Concepts of Biology is designed for the single-semester introduction to biology course for non-science majors, which for many students is their only college-level science course. As such, this course represents an important opportunity for students to develop the necessary knowledge, tools, and skills to make informed decisions as they continue with their lives. Rather than being mired down with facts and vocabulary, the typical non-science major student needs information presented in a way that is easy to read and understand. Even more importantly, the content should be meaningful. Students do much better when they understand why biology is relevant to their everyday lives. For these reasons, Concepts of Biology is grounded on an evolutionary basis and includes exciting features that highlight careers in the biological sciences and everyday applications of the concepts at hand.We also strive to show the interconnectedness of topics within this extremely broad discipline. In order to meet the needs of today's instructors and students, we maintain the overall organization and coverage found in most syllabi for this course. A strength of Concepts of Biology is that instructors can customize the book, adapting it to the approach that works best in their classroom. Concepts of Biology also includes an innovative art program that incorporates critical thinking and clicker questions to help students understand--and apply--key concepts.

Annual Reports on NMR Spectroscopy

Annual Reports on NMR Spectroscopy
  • Author : Graham A. Webb
  • Publisher : Academic Press
  • Release Date : 2010-04-12
  • Total pages : 256
  • ISBN : 9780123813541
  • File Size : 13,8 Mb
  • Total Download : 236
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In recent years, no other technique has gained such significance as NMR spectroscopy. It is used in all branches of science in which precise structural determination is required and in which the nature of interactions and reactions in solution is being studied. Annual Reports on NMR Spectroscopy has established itself as a premier means for the specialist and non-specialist alike to become familiar with new techniques and applications of NMR spectroscopy. * Provides updates on the latest developments in NMR spectroscopy * Includes comprehensive review articles * Highlights the increasing importance of NMR spectroscopy as a technique for structural determination

Aspects of Physical Biology

Aspects of Physical Biology
  • Author : Giancarlo Franzese,Miguel Rubi
  • Publisher : Springer Science & Business Media
  • Release Date : 2008-07-10
  • Total pages : 236
  • ISBN : 9783540787648
  • File Size : 40,9 Mb
  • Total Download : 210
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The application to Biology of the methodologies developed in Physics is attracting an increasing interest from the scientific community. It has led to the emergence of a new interdisciplinary field, called Physical Biology, with the aim of reaching a better understanding of the biological mechanisms at molecular and cellular levels. Statistical Mechanics in particular plays an important role in the development of this new field. For this reason, the XXth session of the famous Sitges Conference on Statistical Physics was dedicated to "Physical Biology: from Molecular Interactions to Cellular Behavior". As is by now tradition, a number of lectures were subsequently selected, expanded and updated for publication as lecture notes, so as to provide both a state-of-the-art introduction and overview to a number of subjects of broader interest and to favor the interchange and cross-fertilization of ideas between biologists and physicists. The present volume focuses on three main subtopics (biological water, protein solutions as well as transport and replication), presenting for each of them the on-going debates on recent results. The role of water in biological processes, the mechanisms of protein folding, the phases and cooperative effects in biological solutions, the thermodynamic description of replication, transport and neural activity, all are subjects that are revised in this volume, based on new experiments and new theoretical interpretations.

Water

Water
  • Author : John L. Finney
  • Publisher : Oxford University Press, USA
  • Release Date : 2015
  • Total pages : 161
  • ISBN : 9780198708728
  • File Size : 37,8 Mb
  • Total Download : 921
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Water dominates the surface of Earth and is vital to life on our planet. It is a remarkable liquid which shows anomalous behaviour. In this Very Short Introduction John Finney introduces the science of water, and explores how the structure of water molecules gives rise to its physical and chemical properties. Considering water in all three of its states as ice and steam as well as liquid, Finney explains the great importance of an understanding of its structure and behaviour to a range of fields including chemistry, astrophysics, and earth and environmental sciences. Finney describes the role of water in biology, and ends with a discussion of of the outstanding controversies concerning water, and some of the 'magical' properties which have been claimed for it. ABOUT THE SERIES: The Very Short Introductions series from Oxford University Press contains hundreds of titles in almost every subject area. These pocket-sized books are the perfect way to get ahead in a new subject quickly. Our expert authors combine facts, analysis, perspective, new ideas, and enthusiasm to make interesting and challenging topics highly readable.